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Image Search Results
Journal:
Article Title: Various Types of Dirofilaria immitis Polyproteins Selectively Induce a Th2-Type Immune Response
doi: 10.1128/IAI.71.7.3802-3811.2003
Figure Lengend Snippet: rDiAg dimers do not induce specific IgE antibody. Mice were administered rDiAg dimers (V2V1 or V1V1), and plasma samples were collected weekly. At second peaks (V2V1 [on day 35] and V1V1 [on day 42]), specific IgE antibodies to dimer (V2V1 and V1V1) and monomer (V1 and V2) were detected by ELISA (A and B) and dot blot analysis (C to E). rDiAg-blotted membranes were treated with serum from mice or rabbits and then reacted with HRP-conjugated anti-mouse IgG (C and E) or anti-rabbit IgG (E). The data represent the mean values for five mice (A and B). The data represent one typical experiment out of five (C to E).
Article Snippet: Total IgG2c and specific IgG levels were measured according to the manufacturer's instructions by
Techniques: Enzyme-linked Immunosorbent Assay, Dot Blot